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Spatiotemporal Distribution of Phenolamides and the Genetics of Natural Variation of Hydroxycinnamoy
發(fā)布時間：2018/1/16 點擊次數：526
Phenolamides constitute a diverse class of secondary metabolites that are found ubiquitously in plants and have been implicated to play an important role in a wide range of biological processes, such as plant development and defense. However, spatiotemporal accumulation patterns of phenolamides in rice, one of the most important crops, are not available, and no gene responsible for phenolamide biosynthesis has been identified in this species. In this study, we report the comprehensive metabolic profiling and natural variation analysis of phenolamides in a collection of rice germplasm using a liquid chromatography–mass spectrometry-based targeted metabolomics method. Spatiotemporal controlled accumulations were observed for most phenolamides, together with their differential accumulations between the two major subspecies of rice. Further metabolic genome-wide association study (mGWAS) in rice leaf and in vivo metabolic analysis of the transgenic plants identified Os12g27220 and Os12g27254 as two spermidine hydroxycinnamoyl transferases that might underlie the natural variation of levels of spermidine conjugates in rice. Our work demonstrates that gene-to-metabolite analysis by mGWAS provides a useful tool for functional gene identification and omics-based crop genetic improvement在這項研究中,我們報告使用基于LC-MS的靶向代謝組學方法在一組水稻種質中對酚酰胺的全面代謝譜和自然變異分析。 大多數酚酰胺的時空控制積累,以及兩大亞種之間的差異積累。 進一步的水稻代謝全基因組關聯研究（mGWAS）和轉基因植物的體內代謝分析將Os12g27220和Os12g27254鑒定為兩種亞精胺羥基肉桂?；D移酶,其可能是水稻中亞精胺結合物水平的自然變異的基礎。 我們的工作表明,由mGWAS進行的基因到代謝物分析為功能基因鑒定和基于組學的作物遺傳改良提供了有用的工具。
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