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LIPASE CATALYZED SYNTHESIS OF ABA-TYPE STRUCTURED LIPID FROM SINGLE CELL OIL AND TRIPALMITIN(脂肪酶催化合成

發(fā)布時(shí)間：2018/3/1 點(diǎn)擊次數(shù)：398

Optimization of lipase-catalyzed interesterification for the production of structured lipid, enriched with arachidonic acid (ARA) from tripalmitin and ARA single cell oil was carried out. Response surface methodology was applied to model and optimize the reaction system; factors of temperature, substrate molar ratio, reaction time and enzyme load were chosen for the optimization. The optimal conditions generated from the models were as follows: temperature of 50C, substrate molar ratio of 1 : 12, reaction time of 13.36 h, and enzyme load of 7.58 wt %. Under these conditions, the incorporation of ARA and acyl migration were 55.1160.15 and 15.8560.05%, respectively. It was found that 69.57% of ARA was mainly incorporated in the sn-1, 3 positions and 62.31% of palmtic acid was located at the sn-2 position of the TAG.(應(yīng)用響應(yīng)面分析法被應(yīng)用于模型和優(yōu)化反應(yīng)系統(tǒng)：溫度,底物摩爾比,反應(yīng)時(shí)間,酶量反應(yīng)條件的優(yōu)化。模型生成的*條件如下:50℃,摩爾比1:12,反應(yīng)時(shí)間13.36h,酶量是7.58wt%。在這些條件下,ARA和酰基位移的結(jié)合分別是55.11±0.15 and 15.85±0.05%。69.57%ARA主要是合并在sn-1, 3位置,62.31%十六酸在sn-2位置。)

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LIPASE CATALYZED SYNTHESIS OF ABA-TYPE STRUCTURED LIPID FROM SINGLE CELL OIL AND TRIPALMITIN(脂肪酶催化合成